=> I am happy to add other academics and vendors weblinks here. conjugated polymer nanoparticles (may need to wait for LinkBright product line to get into Sigma-Aldrich web site to avoid crazy shipping charges from U.K.).
PETER STEELE CRO MAG RALLY PLUS
More on NovaFluor's below (19plex 202111 - 17plex spectra, plus 2 deliberately dimmer variants).ĬPN. based on what I refer to as DNA origami (Duke U.
Some recent fluorophore families based on other technologies: not that this will affect customers too much, though some patent lawyers and law firm(s) likely to be the big financial winners.īrilliants were invented by Sirigen (acquired by BD Biosciences), which was cofounded by 2000 Chemistry Nobel laureates to commercialize their discovery of electron carrying polymers. StarBright Ultraviolet (340nm excitation max, should excite at 368nm wrt FISHscope], StarBright Violet, StarBright BlueĪbove are, or likely, "polymer dyes", based on Nobel Prize in Chemistry 2000, (Sirigen was acquired by BD Biosciences - more on this below). See below for possible Alexa Fluor acceptors of BV's. BD Biosciences (some BVs available BioLegend, Jackson Immunores). Repear with Brilliant Biolets, SBVs, etc.īrilliant Ultraviolets, Violets, Blues, Yellow-Green(s). Easiest if the different markers are in different locations, such as different cells.įor example, may be able to multiplex ALL the BD Brilliant Ultraviolets (BUV's) with Bio-Rad's StarBright UltraViolets (SBUVs) and add in fluorescent quantum dots (QDots). GM Suggestion = Hypothesis: Could Multiplex different brands using the same excitation and emission - especially if you have the right microscope, such as a spectral fluorescence lifetime imager (you could buy the image core a fully loaded Leica STELLARIS 8 with 5 HyD S SiPMs inside, and 4 more on X1 port, for us to figure out - other brands&models possible), and right software. MPMicro is available online at (please do not hit the print button).īelow is a summary I posted on linkedin and add some more text here - the linkedin page has some graphics of spectra (we have hit a limit on our web hosting site * budget wrt graphics here), see also and other spectra search web sites, or if you prefer Microsoft Excel data, my (2006) PubSpectra data set is at G M Suggestion = Hypothesis: Could Multiplex different brands I have a long standing interest in multiplex - aka multi-Probe - fluorescence microscopy, having started "MPMicro" for a lecture at the Nikon AQLM course at MBL in 5/1995 (26 years ago on starting this page 5/2021). This page mentions a few publications (by others) I think are useful (ex: Ce3D and IBEX from ron Germain's lab).
Goal: maximize the amount of useful information, in as few stain-image-destain cycles as possible (ideally N=1), from each cell or 2D tissue section (most common(, or 3D tissue or organoid or whole-mount (organ, embryo, larva, adult animal ).
Some alternative terms: fluorescence image cytometry, spatialomics, spatial genomics (usually single molecule RNA, can be DNA), spatial proteomics. 20210512W (start date) note: Multiplex Microscopy
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